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After adding 0.4 ml of 1 M sodium citrate buffer, pH 6.0, the mixtures were incubated at 37 C for 10 min with shaking at 125 rpm on a rotary shaker NR-3 (TAITEC) to facilitate release of bound sulfur as H 2 S gas from the aqueous phase

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In view of this finding, the protective effect of ROF against MTX-induced liver damage was mediated by its anti-apoptotic effects