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Design and synthesis of glutathione analogues

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The proteins were electroblotted onto a PVDF membrane (Roth, Karlsruhe, Germany)

Then colonoids were stained with 1 g/mL BODIPY 493/503 (Thermo Fisher Scientific, #D3922) in PBS for 1 h at RT, washed twice in PBS, and incubated with Phalloidin as described above

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