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However, the GPI-anchoring module allows the GST fusion to express in the endoplasmic reticulum (ER) lumen and translocate to the out leaflet of the cell membrane through the Golgi apparatus ( In brief, we develop a gene transfection-positive cell sorting system utilizing GPI-anchored GST as the affinity tag that enables positive cell isolation using GSH magnetic beads

A sterile environment is also non-negotiable

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Intramuscular injection Some protocols specify intramuscular rather than subcutaneous administration