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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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Patients in their 40s and 50s who have never addressed the GHK-Cu decline often present with compounded effects: skin that has thinned significantly, hair that has changed in texture and density over years, and a recovery timeline after procedures that is noticeably longer than it was in their 20s

doi: 10.1097/CM9.0000000000002533 17 DixonS

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A B12 injection bypasses the digestive tract, ensuring direct absorption into the bloodstream