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Dermal fibroblast proliferation, keratinocyte signaling, and wound-closure assays

Amino acids V12 (putative oxyanion hole), F112 (active site architecture), N108 (flavonoid binding) and C13 (GSH stabilization) were fully randomized by site saturation mutagenesis (NNK codons), and the created Pt GSTF8 variants were tested for conversion of flavan-3,3,4-triol ( 4 ) to cyanidin ( 8 ), and for their GSH-transferase activity towards CDNB ( 5 ) as a control of catalytic competence

These generally show continued safety, but definitive long-term studies don't exist

Prefer topical glutathione serums or creams over high-dose intravenous (IV) glutathione in unregulated settings

Vitamin D injections : Therapeutic doses vary but often include amounts like 50,000 IU for treating deficiencies

doi: 10.1038/s41598-022-12086-8 99