For LC-MS, the reaction was prepared with 10 M enzyme, 200 M GTP in 20 mM HEPES buffer, pH 6.8, 150 mM NaCl, 10 mM MgCl 2 .The reaction was carried out at 30 C for 5 min, then deactivated at 95 C for 5 min
It is chemically a true peptide (six amino acids, including two D-amino acid substitutions for stability), so unlike some non-peptide GH secretagogues it is correctly classified in this category
The purchase was a brief, the shipping was fast and easy, packet arrived complete well package with instructions and all the necessary items to start
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