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The enzyme extraction buffer consisted of HEPES buffer (pH 7.8) supplemented with 20% (v/v) glycerol, 1 mM EDTA, 1 mM AsA, 1 mM glutathione, 5 mM MgCl, and 1 mM DTT

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Further evidence for the physiological relevance of the GST isomerase activity derives from the coregulation of dehydrogenase and GSTA3 gene transcription in the horse (Loux et al., 2020)

Selenium: Synergistic selenium is required for glutathione peroxidase activity