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As an NNMT inhibitor, it is associated with reduced white adipose tissue, improved NAD+ availability, and support for insulin sensitivity
NAD+ metabolism enzyme NNMT in cancer-associated fibroblasts drives tumor progression
Sample preparation serum (lithium heparin) A volume of 25 l of serum were mixed with 250 l a cold solvent mixture with ISTD (methanol/water/chloroform, 9:1:1, 20 C), into 1.5 ml microtube, vortexed and centrifugated (10 min at 15,000 g , 4 C)