Images were minimally processed for brightness-contrast adjustments using Adobe Photoshop 7.0
Then, the medium was changed to fresh DMEM containing DCFH-DA (10 M) and Hoechst 33342 for ROS analysis and nuclei stain
To keep Glutaryl Glutathione Spray effective
Testis-specific transcriptional control
Abbreviations GCLC: Glutamate cysteine ligase catalytic subunit GCLM: Glutamate cysteine ligase modifier subunit GSH: Reduced glutathione GSSG: Oxidized glutathione ROS: Reactive oxygen species C1q: Complement component 1q C3: Complement component 3 GSDMD: Gasdermin D GSDME: Gasdermin E DAM: Disease associated microglia DAA: Disease associated astrocyte Iba1: Ionized calcium-binding adapter molecule 1 GFAP: Glial fibrillary acidic protein NeuN: Neuronal nuclei HO1: Hemeoxygenase 1 CCL: CC Chemokine ligand CXCL: C-X-C motif ligand AD: Alzheimers disease App: Amyloid precursor protein A: Amyloid beta App : NL-G-F App knockin mouse NL-G-F References Guo, C., Sun, L., Chen, X
Melanie Avalon Just my personal perception was, again, I haven't done the IV, but when I was doing the injections, it didn't feel good