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Experiments are designed to quantify its effects on collagen I and III synthesis (via qPCR or Western blot), myofibroblast differentiation (by measuring -smooth muscle actin expression), and the critical MMP/TIMP balance

Gut microbes promote colonic serotonin production through an effect of short-chain fatty acids on enterochromaffin cells

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Thus, data in support of each hypothesis was derived from different animal models, with mitochondrial oxidative stress being critical for APAP hepatotoxicity in mice on a normal diet, without appreciable LPO generation