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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

Its engineered amino acid substitutions, fatty-acid acylation, and carefully balanced receptor affinity work together to improve stability, prolong circulation, and enable coordinated investigation of multiple metabolic pathways within a single experimental compound

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This disorder is most correctly referred to as carnitine deficiency, systemic primary (CDSP)