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At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS

Individual response varies based on age, baseline hormone levels, lifestyle factors, and dosing protocol

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Biogenic fabrication of iron oxide nanoparticles from Leptolyngbya sp