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Cell viability and proliferation were determined using the CCK-8 kit (MA0218, Meilun) at 0, 24, 48, 72, and 96 h post seeding, as described previously (Yang et al., 2022)
Mitochondrial glutathione depletion is directly linked to oxidative damage to mitochondrial DNA, impaired cellular energy production, and the patterns of age-related functional decline observed across tissues
As described above, TGF--induced HK-2 cells are accompanied by ferroptosis activation, and HK-2 cells treated with TGF- also presented increased protein and mRNA levels of fibronectin, collagen-1, and -SMA
Research Limitations 3
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