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For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

Cypess, A

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When your stomach takes more time to empty itself of food, it sends signals of fullness to the brain

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Some people may feel appetite changes