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After labeling, cells were gently quenched, washed three times with DPBS, fixed and permeabilized with ice-cold methanol for 10 min

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Aim for at least one liter of water per day , and more if engaging in physical activity

Next, an orthotopic K7M2 osteosarcoma model with an active immune system was established to investigate the tumor targeting and therapeutic effects of NP4 (Fig