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Samples were mixed with a nondenaturing PAGE loading buffer containing 5% (v/v) glycerol, bromophenol blue and Trisborate (TB) buffer (100 mM Tris and 100 mM boric acid) and run on a native PAGE gel (prerun at 200 V for 5 min) for 50 min (130 V, 4 C) in TB buffer
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However, this comes with significant caveats
The most critical factor determining longevity is storage temperature - every 10C increase roughly doubles degradation rate, making freezer storage (-20C) essential for long-term powder storage and refrigeration (2-8C) mandatory for reconstituted peptides
