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cAMP accumulation HEK 293T cells were cultured in Dulbeccos Modified Eagles Medium (DMEM) (Gibco, Waltham, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco) and 1% (v/v) sodium pyruvate (Gibco) at 37 C, 5% CO 2 in a humidified incubator
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Kolkhof et al
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Since GHS is orally bioavailable, the logic for this application was that it could be added in relatively large amounts to feed for dairy cattle
However, both have potential gastrointestinal side effects, which can be amplified if an individual taking the prescription drug prednisone decides to also take ibuprofen
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