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Sivalingam S, Larsen EL, van Raalte DH, Muskiet MHA, Smits MM, Tonneijck L, et al
Nevertheless, response patterns exhibit considerable individual variability, with weight loss ranging from 0% to over 10% among different patients
After a further centrifugation (200 x g, 8 min, 20C, Universal 320, Andreas Hettich GmbH, Tuttlingen, Germany), PBMC were resuspended in RPMI medium supplemented with fetal bovine serum, HEPES buffer, L-glutamine, and -mercaptoethanol (all purchased from Biochrom AG, Berlin, Germany)
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