Improved Insulin Sensitivity Insulin sensitivity refers to how well the body uses glucose
After 96 h, wells were aspirated, washed with PBS, and a fixative/staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399) was added
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The reaction solution gradually becomes black, stirred at room temperature for 3 h, the solvent is concentrated to 10 mL by distillation under reduced pressure