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Acylcarnitine assay Acylcarnitines were extracted from either 10 l of whole liver homogenate, 50 l of plasma, 50 l brain parenchyma, 50 l cerebrovasculature (timepoint study), 60 l of cerebrovasculature (cerebrovascular uptake study) or 100 l of brain homogenate (all homogenized as described above) and spiked with 5 l of acylcarnitine internal standard (IS) mix containing 12 M TMAO-d9 (Cambridge Isotope Laboratories, Inc., CIL), NSK-B (CIL) and NSK-B-G1 (CIL) at the beginning of the extraction process
Liquid chromatography combined with spectrophotometric detection and mass spectrometry was also rarely used
SzutowiczAMadziarBPaweczykTTomaszewiczMBielarczykH
PMC 4953733
Ultimately it leads to an infiltration of he thyroid with lymphocytes (immune cells), scarring and destruction of the thyroid gland
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