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The proteome concentration of lysates was normalized to 2 mg/mL, and 100 L was transferred to a LoBind Eppendorf tube containing 48 mg of urea

Aging Clin Exp Res

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The ESP-EORTC-EURACAN recommendations concerning Spitz melanoma should be applied also on the basis of the recent observation that a spitzoid morphology is not invariably associated with a Spitz genetic signature ( Figure 6 illustrates the clinicopathological features of an ulcerated melanocytic malignancy histopathologically composed of large epithelioid cells with Spitz-like features, but immunohstichemically typified as a classical melanoma because of its immunohistochemical positivity to the anti-BRAF mutated protein VE1 antibody

The protein concentration was determined with the Bradford protein assay (Bio-Rad)

Mod Pathol