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Recombinant proteins in the supernatant were purified using nickelSepharose resin (GE Healthcare) and initially washed with wash buffer B1 (50 mM Tris, 500 mM NaCl and 30 mM imidazole, pH 8.0) until no protein was detected in the eluent using the Bradford reagent

There are still three other levers of attack against DHT

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for sensitive skin, start with once daily use and perform a patch test on a small skin area

After amplification, PCR products were electrophoresed on agarose gels and transferred onto nylon membranes using Southern blotting techniques