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The mechanism for the observed hypometabolism is still unclear, although we believe that reduced glucose uptake is likely, as in our mouse pilocarpine model hexokinase activity appeared to be unchanged in hippocampus ( In addition, lactate concentrations are often elevated during and between epileptic seizures in human epileptic foci ( In several animal models increases in brain lactate levels have been found after a period of seizure activity ( A receptor channels) in young and adult mice or the initiation of kainate-induced status epilepticus (SE) in rats there was an increase in the levels of hippocampal and brain lactate, respectively (Yang et al., 2013) and will not be discussed here
Samples were characterized using three independent platforms: ultrahigh-performance liquid chromatography with tandem mass spectrometry (UPLCMS/MS) in the negative-ion mode, UPLCMS/MS in the positive-ion mode and gas chromatographymass spectrometry (GCMS) after sialylation
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J Am Chem Soc 125:1345513460
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