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Since MTX induces oxidative stress by increasing reactive oxygen species in tissues (Reference Jahovic, Cevik and Sehirli38,Reference Moghadam, Tutunchi and Namvaran-Abbas-Abad40,Reference Olayinka, Ore and Adeyemo43) , the effects of various antioxidant substances against MTX-induced oxidative stress have been investigated (Reference Savran, Cicek and Doguc7,Reference Jahovic, Cevik and Sehirli38,Reference Olayinka, Ore and Adeyemo43Reference Yuksel, Yuksel and Yagmurca45)

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Glutathione supplementation should never replace evidence-based lifestyle interventions, which remain the primary treatment for fatty liver disease

Adequate hydration, fiber supplementation, and physical activity address the issue in most cases

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Free GHK does not display the same metal-mediated biochemical interactions and is generally less stable in aqueous environments