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The cells were exposed for various times, after which the media was removed and the cells were washed with warm PBS and lysed by brief sonication in fresh PBS
Briefly, the brain homogenate was diluted 1:20 (v:v) with ice-cold Lockes buffer (154 mM NaCcl, 5.6 mM KCl, 3.6 mM NaHCO3, 2.0 mM CaCl2, 10 mM D-glucose and 5 mM HEPES, pH 7.4) to obtain a concentration of 10 mg tissue/ml
doi: 10.3390/ijms14058801 4 WilsonMRKugelSHuangJWilsonLJWloszczynskiPAYeJet al
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