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SD rat Six- to 7-week-old male SD rats (Charles River Laboratories) were fed a HFD for a total of 5 weeks and dosed with vehicle (5% DMSO and 0.5% Kollidon-30 in 100 mM sodium phosphate buffer) or TLC-2716 (0.1 or 1 mg per kg (body weight)) once daily by oral gavage for the last 3 weeks

Injecting on a day when you can prioritize hydration and rest, rather than a busy workday, gives your body more support during the peak side-effect window

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