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Description

This phenomenon can be explained by compounds that have little to no activity, helping the main active component reach its target through mechanisms of action that complement each other, reverse resistance, improve bioavailability, reduce metabolism and excretion, and modulate side effects (93)

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Proteins are sensitive to heat, ions (acids, bases, metal ions), organic solvents, and other external factors, and are prone to denaturation, aggregation, and precipitation reactions, thereby losing their biological activity

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Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min

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