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Guideline for the diagnosis and management of glutaryl-CoA dehydrogenase deficiency (glutaric aciduria type I)

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After treated with test compounds for 15 hours, harvested in 5 ml Dulbecco s modified medium containing Deep Red Reagent (5 M) (Molecular Probes, Invitrogen) and incubated for 30 min at 37C in a tissue culture incubator