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additionally NAD + can be generated in the mitochondria from NMNAT3 80 , whose activity has been shown to be required in cells 77 , but dispensable in vivo as NAD + can be replenished through other pathways such as import from the cytosol 81

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This system-wide dysfunction in mice fed with choline-deficient choline was observed in the circulation, as it modulates plasma proteins associated with inflammation, immune response, and metabolic processes, including insulin metabolism, mitochondrial function, inflammation, and fructose metabolic processing (163)

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