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In brief, the cycling mix was made fresh immediately before the assay, 5 l of diluted supernatant or NAD + standard was mixed with 95 l of cycling mix, which is made with 2% ethanol, 20 M resazurin, 10 M flavin mononucleotide, 10 mM nicotinamide, 0.1% BSA in 100 mM sodium phosphate buffer, 100 g ml 1 alcohol dehydrogenase and 10 g ml 1 diaphorase
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