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& Murlin, J

Ingredients 1 tbsp milk thistle seeds 1 tbsp seaweed flakes 1 tbsp dried nettle leaves 1.5 tsp celery seeds 5 tbsp unrefined sea salt How to make milk thistle salt Place the milk thistle seeds, seaweed, nettle and celery seeds into a spice or coffee grinder and grind to preference

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Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h