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For each differential gene expression analysis, a pseudo-bulk gene expressed matrix was generated by summing the transcript counts for all cells with the same cell type and mouse combination

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While the C-terminus of oxyntomodulin and the receptor ECD/ECL1 was lower resolution in the cryo-EM map, the interaction patterns in the modelled protein and the MD simulations were largely similar to GLP-1, albeit that the nature of some interactions differed due to their differing sequences