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Fifty microliters of standard solutions were mixed with assay cocktail (12.5 l of 4 mM NADPH in 5% NaHCO 3, 25 l of GSH reductase solution (Roche, No.105678, 5 mg/ml), 12.5 l of 10 mM 5,5-dithiobis-2-nitrobenzoic acid in 0.1 M phosphate buffer, 75 l MES buffer, 25 l distilled water) on 96-well plate
What is NAD
It can also be difficult to determine the source of the collagen and its purity
The combination approach of targeting two separate pathways produces results competitive with even the most effective single-agent therapies currently available
Donald D
Maple, J
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