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In RBL-2H3 cells, apigenin treatment (30 M) also inhibited these signaling molecules, and the expression of FcR1 and cytokines TNF-, IL-4, IL-5, IL-6, IL-13, and COX-2 [135]

For most research protocols, 4-6 sprays per day are needed

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Mice were euthanized three-and seven-weeks post infection for juvenile and adult worms, respectively, using a lethal dose of 0.018 ml of Euthasol and 5.85 mg/ml heparin to prevent blood coagulation (injection volume of 400 l)