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10 Discussion and future directions While the compelling evidence underscores the immense potential of miRNAs as sensitive diagnostic biomarkers for non-invasive liquid biopsy-based staging and multi-target therapeutic agents capable of modulating key hyperglycemia-induced pathways (oxidative stress, inflammation, angiogenesis, neurodegeneration) in DR, significant translational challenges necessitate focused future research, including rigorously validating the diagnostic accuracy, specificity, and predictive power of candidate miRNAs across diverse, large-scale longitudinal cohorts and ethnic populations using standardized, sensitive profiling techniques like ddPCR to overcome limitations of variability and establish clinically actionable thresholds

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As fitting function, the following equation was used where M z (t) is the longitudinal relaxation at the recovery delay time t , M 0 is the equilibrium magnetization and R i is either the longitudinal relaxation rate R 1 or R 1

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For information on recommended timing of when to stop GLP1-RAs prior to pregnancy please see Glucagon-like peptide-1 receptor agonists during pregnancy and lactation