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It is possible that the lower ghrelin levels in the obese may be a consequence of overconsumption, instead of the cause of the signal to other brain regions

Potassium phosphate buffer, EDTA, sodium azide, oxidized glutathione (GSSG), and bovine serum albumin (BSA) were added to the Liver homogenate supernatant

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This modification makes the molecule more stable in the gut and allows it to pass through the cell membrane more effectively

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