The tissue powder was suspended in four volumes of cold (4C) extraction solution which contains 5% (w/v) stabilized metaphosporic acid (Acros Organics, USA) and 1 mM EDTA in 0.1% formic acid, supplemented with 1% (w/v) polyvinyl-polypyrrolidone just before use
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Uncropped western blots are provided in the Supplementary Information
Xu RY, Wang DH, Wang CL, Zhang GC, Wei GY (2018) Improved S-adenosylmethionine and glutathione biosynthesis by heterologous expression of an ATP6 gene in Candida utilis