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Briefly, HCT116 p53+/+ cells were collected, washed with PBS and diluted with cell lysis buffer (25 mM Tris-HCl (pH 7.5) and 10 mM MgCl 2 ) supplemented with complete protease inhibitor cocktail
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Adherence concerns: Many people stop treatment early, leading insurers to question the long-term return on investment for these medications
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