Briefly, 100 L of cell culture medium from ECFCs were mixed with an equal volume of Griess reagent (1% sulfanilamide, 0.1% N-1-naphthalenediamine dihydrochloride, and 2.5% H 3 PO 4 ) and transferred to 96-well plates
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Within the acidic lysosome, the imidazole groups of histidine residues become protonated, which both buffers the organelles pH and induces membrane destabilization, thereby driving the escape and cytosolic release of encapsulated paclitaxel [28]