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During this process, NH 3 is released and the lipoyl group of the H protein becomes reduced (dihydrolipoyl form)

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First, the target RNA was folded by adding 1 PKN buffer (20 mM phosphate, 80 mM KCl, and 20 mM NaCl at pH 7.0), followed by heating at 95 C for 5 min and cooling on ice

Then, once a week for four more weeks

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