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Since 90% of the bodys serotonin originates in the gut, modulating enteric serotonin production could influence central serotonergic tone through vagal afferents that relay gut signals to brain serotonin-producing raphe nuclei

Start based on symptoms and goals

Standard reconstitution uses 2 mL of bacteriostatic water per 5 mg vial, producing a concentration of 2.5 mg per mL or 0.25 mg per 0.1 mL (10 units on an insulin syringe)

However, it is important to always consult with a medical professional before taking B12 injections as they could affect the efficacy and safety of other medications

Nicotinamide riboside

If you are particularly sensitive to new supplements, you can start by taking it in the morning to see how your body reacts